2022년 The 28th National Congress of Digestive Diseases (Digestive and Liver Disease 54S2 (2022) S85. OC.07.2)
Background and aim: Autoimmune atrophic gastritis (AAG) is an organ-specific disease characterised by progressive atrophy of the gastric body and fundus mucosa, with subsequent hypo-achlorhydria. To date, no therapy proved effective in healing or improving mucosal inflammation and atrophy in AAG. Zinc l-carnosine is a chelate compound of zinc and l-carnosine, which has shown anti-inflammatory and anti-apoptotic properties in several conditions, and it is currently being used for peptic ulcer disease. We here report the results of ex vivo experiments conducted by culturing AAG gastric biopsies with zinc l-carnosine.
Materials and methods: Perendoscopic gastric corpus biopsy specimens from 18 AAG patients (median age 59 years, 12 females; all having severe gastric atrophy) were placed on iron grids in the central well of an organ culture dish and the dishes placed in a tight chamber with 95% O2/5% CO2 at 37°C. Biopsies were cultured for 24 hours in serum-free HL-1 medium (Cambrex Bio Science, Milan, Italy), added with antibiotics, with or without 10 ng/mL zinc l-carnosine. After 24-hour culture, the concentrations of TNF-α, IL-15, IFN-γ, IL-17, IL-6, and IL-21 were measured in organ culture supernatants using the Luminex x-MAP technology (Luminex Corporation, DiaSorin Company, Austin, TX, USA).
Results: The Figure shows levels of cytokines, expressed in pg/mL, in the supernatants of organ culture gastric corpus biopsies. Of note, zinc l-carnosine significantly down-regulated the production of IFN-γ, TNF-α, IL-21, IL-6, and IL-15, while no significant effect was observed for IL-17.
Conclusions: Zinc L-carnosine was able to down-regulate several pro-inflammatory cytokines produced by AAG gastric biopsies cultured ex vivo. The prolonged suppression of inflammation within the gastric mucosa could potentially improve, over time, gastric lesions, or prevent disease progression. Long term in vivo studies are needed to confirm this assumption.
2022년 The 28th National Congress of Digestive Diseases (Digestive and Liver Disease 54S2 (2022) S85. OC.07.2)
Background and aim: Autoimmune atrophic gastritis (AAG) is an organ-specific disease characterised by progressive atrophy of the gastric body and fundus mucosa, with subsequent hypo-achlorhydria. To date, no therapy proved effective in healing or improving mucosal inflammation and atrophy in AAG. Zinc l-carnosine is a chelate compound of zinc and l-carnosine, which has shown anti-inflammatory and anti-apoptotic properties in several conditions, and it is currently being used for peptic ulcer disease. We here report the results of ex vivo experiments conducted by culturing AAG gastric biopsies with zinc l-carnosine.
Materials and methods: Perendoscopic gastric corpus biopsy specimens from 18 AAG patients (median age 59 years, 12 females; all having severe gastric atrophy) were placed on iron grids in the central well of an organ culture dish and the dishes placed in a tight chamber with 95% O2/5% CO2 at 37°C. Biopsies were cultured for 24 hours in serum-free HL-1 medium (Cambrex Bio Science, Milan, Italy), added with antibiotics, with or without 10 ng/mL zinc l-carnosine. After 24-hour culture, the concentrations of TNF-α, IL-15, IFN-γ, IL-17, IL-6, and IL-21 were measured in organ culture supernatants using the Luminex x-MAP technology (Luminex Corporation, DiaSorin Company, Austin, TX, USA).
Results: The Figure shows levels of cytokines, expressed in pg/mL, in the supernatants of organ culture gastric corpus biopsies. Of note, zinc l-carnosine significantly down-regulated the production of IFN-γ, TNF-α, IL-21, IL-6, and IL-15, while no significant effect was observed for IL-17.
Conclusions: Zinc L-carnosine was able to down-regulate several pro-inflammatory cytokines produced by AAG gastric biopsies cultured ex vivo. The prolonged suppression of inflammation within the gastric mucosa could potentially improve, over time, gastric lesions, or prevent disease progression. Long term in vivo studies are needed to confirm this assumption.