2022년 The 28th National Congress of Digestive Diseases (Digestive and Liver Disease 54S2 (2022) S73. OC.03.1)
Background and aim: The extracellular nicotinamide phosphoribosyltransferase (eNAMPT), characterized by a pro-inflammatory activity, is overexpressed and oversecreted in inflammatory bowel diseases. However, nothing is known about the role of eNAMPT in other gastrointestinal disorders, such as autoimmune gastritis (AIG) which is characterized by an activation of several inflammatory pathways within the gastric mucosa. We aimed to explore the role of eNAMPT in gastric organ cultures from patients with AIG.
Materials and methods: To study the expression of NAMPT in AIG organ cultures, we collected biopsies with Jumbo forceps during upper GI endoscopy from the gastric corpus mucosa of AIG patients, as well as from age and sex matched healthy controls (i.e., patients with no gastric alterations). First, biopsies from AIG (n=10; median age 56 years, 7 females) were processed for RT-qPCR to assess the mRNA expression of NAMPT compared to healthy controls (n=10; median age 51 years, 7 females). Furthermore, biopsies from the 10 healthy subjects were weighed and plated in vitro as explants and stimulated with NAMPT (500 ng/mL). After 24 hours, supernatants were collected for the detection of a panel of cytokines (i.e., TNFalfa, IL-8, IL-6, IFNgamma, TGFbeta, IL-33, IL-1, IL-11) and eNAMPT, by using Bio-plex technology.
Results: Compared to healthy controls, we found an increase in the mRNA expression of NAMPT in biopsies from patients with AIG (Figure) that was not paralleled by an increase in eNAMPT serum levels. We also observed that the exposure of human biopsies from healthy controls to eNAMPT led to a significant increase in the mRNA expression of several pro-inflammatory or regulatory cytokines, including IL-8, IL-6, IFNgamma, TGFbeta, IL-33 (Figure), thereby indicating that NAMPT may play a role in determining the inflammatory processes within the gastric mucosa.
Conclusions: Our data indicate that eNAMPT may act as a proinflammatory cytokine in AIG and could pave the way to further studies investigating this molecule as a potential therapeutic target and inflammatory biomarker in this condition.
2022년 The 28th National Congress of Digestive Diseases (Digestive and Liver Disease 54S2 (2022) S73. OC.03.1)
Background and aim: The extracellular nicotinamide phosphoribosyltransferase (eNAMPT), characterized by a pro-inflammatory activity, is overexpressed and oversecreted in inflammatory bowel diseases. However, nothing is known about the role of eNAMPT in other gastrointestinal disorders, such as autoimmune gastritis (AIG) which is characterized by an activation of several inflammatory pathways within the gastric mucosa. We aimed to explore the role of eNAMPT in gastric organ cultures from patients with AIG.
Materials and methods: To study the expression of NAMPT in AIG organ cultures, we collected biopsies with Jumbo forceps during upper GI endoscopy from the gastric corpus mucosa of AIG patients, as well as from age and sex matched healthy controls (i.e., patients with no gastric alterations). First, biopsies from AIG (n=10; median age 56 years, 7 females) were processed for RT-qPCR to assess the mRNA expression of NAMPT compared to healthy controls (n=10; median age 51 years, 7 females). Furthermore, biopsies from the 10 healthy subjects were weighed and plated in vitro as explants and stimulated with NAMPT (500 ng/mL). After 24 hours, supernatants were collected for the detection of a panel of cytokines (i.e., TNFalfa, IL-8, IL-6, IFNgamma, TGFbeta, IL-33, IL-1, IL-11) and eNAMPT, by using Bio-plex technology.
Results: Compared to healthy controls, we found an increase in the mRNA expression of NAMPT in biopsies from patients with AIG (Figure) that was not paralleled by an increase in eNAMPT serum levels. We also observed that the exposure of human biopsies from healthy controls to eNAMPT led to a significant increase in the mRNA expression of several pro-inflammatory or regulatory cytokines, including IL-8, IL-6, IFNgamma, TGFbeta, IL-33 (Figure), thereby indicating that NAMPT may play a role in determining the inflammatory processes within the gastric mucosa.
Conclusions: Our data indicate that eNAMPT may act as a proinflammatory cytokine in AIG and could pave the way to further studies investigating this molecule as a potential therapeutic target and inflammatory biomarker in this condition.